Query Information

ORIGIN plant
BEGIN
>MYSEQ
 CDIIDGTYNI HMFQIVYLLC KKTLNKTLIS KTQETKILNH TYCILFLTNL
 EDEIIFKGVG FVTPEIFWGI IIEIKVKCPI VILWNPPSSK NKKHNNISGK
 IEIEIQFVNF KKMDYPLLGL YIKYFFTCSN SKKNTLLPPK IIKIFTFKNF
 NNTNHIFLKI NISCVHCIFE INFQITRDNK KYLHIILDIL SVCCEFYSLK
 PRIKIEVGIN NGKIRNIKKK KK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          222 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 66 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 73   Charge diffirence(C-N):  4.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   9
     Peak Value of UR:   2.10
     Net Charge of CR: -1
     Discriminant Score:     -1.45
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -6.43
     Possible cleavage site: 50
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -2.18  threshold: -2.0
     INTEGRAL    Likelihood = -2.18   Transmembrane   66 -  82 (  66 -  83)
     PERIPHERAL  Likelihood = -0.37
     modified ALOM score:   0.54
>>> Seems to be a Type Ib (Nexo Ccyt) membrane protein
     The cytoplasmic tail is from 83 to 222 (141 Residues)
( 1) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 1
     Uncleavable? Ipos set to: 11
Discrimination of mitochondrial target seq.: 
     negative (-2.61)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 13.39 at (69)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  3.86
     score from the 1-31 region:  2.95
Chloroplast protein?  Status: negative (-4.15)

*** Reasoning Step: 2

> Relative position of the end of the tail: 37%
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.107
SKL motif (signal for peroxisomal protein): 
     pos: -1(222), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -0.47
Peroxisomal proteins?   Status: negative
     AAC score (peroxisome): 0.005
Amino acid composition tendency for vacuolar proteins
     Score:  5.53  Status: positive
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 217 (5) KKKK
     Found: pos: 218 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
     Found:  pos:  86 (3) PSSKNKK
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
     RNP motif found:  pos.  56 (6) KGVGFVTP
nuc modified.   Score:  0.70
Nuclear Signal   Status: notclr ( 0.40)
Check cytoplasmic tail for typeIb (plasma memb.)
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                          nucleus --- Certainty= 0.460(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.200(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.107(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.100(Affirmative) < succ>


----- The End -----