Query Information

ORIGIN plant
BEGIN
>MYSEQ
 CARPPWPKRP LSGFSPATSA KIHRTVRCAP DCPVSQRSAG PTVGRVIGAR
 HVAEPTVGGV HRTVRCARRQ KARIAFQMCS QRLLGALGLK GPLGAWRSTT
 KHSYNLSKHQ DIDLAHSIHC DSISSCGVVY SLSCVASSYC CLCACCSDLL
 KSCVRRSFPP LLRDSLTSFV RARGSKLWRF LASGIEKRKA RTPWYSSSLD
 HLRGVECNSR PLGRHNVEVG KFCTWPNHGI KKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          234 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 128 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 135   Charge diffirence(C-N):  2.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   11
     Peak Value of UR:   0.51
     Net Charge of CR: 3
     Discriminant Score:     -9.26
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -2.07
     Possible cleavage site: 20
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  -0.59  threshold: -2.0
     PERIPHERAL  Likelihood = -0.59
     modified ALOM score:  -0.78
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 99
     WRSTTK
Discrimination of mitochondrial target seq.: 
     negative (-1.75)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.24 at (55)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -1.85
     score from the 1-31 region:  6.79
Chloroplast protein?  Status: negative (-7.19)
Res.2 is Ala (a feature for chloroplast seq.)
Res.2 is Ala (a feature for chloroplast seq.)
Res.2 is Ala (a feature for chloroplast seq.)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: 175(234), count: 1   SKL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:  -1.51
Peroxisomal proteins?   Status: notclr
Amino acid composition tendency for vacuolar proteins
     Score: -3.53  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 230 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.60
Nuclear Signal   Status: notclr ( 0.30)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

           microbody (peroxisome) --- Certainty= 0.300(Affirmative) < succ>
                          nucleus --- Certainty= 0.300(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.280(Affirmative) < succ>
               chloroplast stroma --- Certainty= 0.200(Affirmative) < succ>


----- The End -----