Query Information

ORIGIN plant
BEGIN
>MYSEQ
 ARRLEVEEIP HTGVLSISIL SLASGIIFCR YASYGWCELA YHPATGLDGR
 MGCCSDRRGA GGVVGKALPS LLSTISIICC SLFCELISVA EKLFMFQYFK
 LRFSCFIVFL LLTFEVCGIS WLSGFIRLDL RDRVIPIVCY GPGVTALKLE
 FGRSATPGLR GLLNGEVLRN R

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          171 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 3
     Position of the most N-terminal TMS: 13 at i=3
MTOP: membrane topology (Hartmann et al.)
     I(middle): 20   Charge diffirence(C-N):  0.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   18
     Peak Value of UR:   2.35
     Net Charge of CR: 0
     Discriminant Score:      3.71
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 2.62
     Possible cleavage site: 25
>>> Seems to have a cleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 26
ALOM new cnt: 3 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 1B
ALOM: finding transmembrane regions (Klein et al.)
     count: 2  value:  -6.74  threshold: -2.0
     INTEGRAL    Likelihood = -6.74   Transmembrane  103 - 119 (  98 - 126)
     INTEGRAL    Likelihood = -5.31   Transmembrane   71 -  87 (  70 -  91)
     PERIPHERAL  Likelihood =  3.61
     modified ALOM score:   1.45
>>> Likely a Type IIIa membrane protein (clv)
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 5
     RRLEVE
Discrimination of mitochondrial target seq.: 
     negative (-0.82)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.96 at (66)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.03
     score from the 1-31 region:  7.47
Chloroplast protein?  Status: negative (-4.99)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: -1(171), count: 0   
Amino Acid Composition Tendency for Peroxisome:   3.97
     AAC not from the N-term., score modified
Peroxisomal proteins?   Status: notclr
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score:  2.21  Status: positive
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.640(Affirmative) < succ>
                       Golgi body --- Certainty= 0.460(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.370(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>


----- The End -----