Query Information

ORIGIN plant
BEGIN
>MYSEQ
 ARQATFPIRS EILSISSAKQ IVQHGHYIKK NLLVVYEPSP IKASYSYNGA
 AQSFTAVVLN LVIGYGSFSA LMSNRDQANM LPRGSDLPDL VLVLTEVVDW
 RRGPHHTLDR SEAKDQYQSL PPSQPCGYPF LRRSSDKIGY TRKAARQLVR
 KQNHWSKIPI KAERSHRKKH IQEKRQAGAA SPKAEREKLG SSSTDLVVDS
 HRGSSANLSM EVG

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          213 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 56 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 63   Charge diffirence(C-N): -3.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   7
     Peak Value of UR:   1.09
     Net Charge of CR: 1
     Discriminant Score:     -7.80
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -3.02
     Possible cleavage site: 43
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  -1.44  threshold: -2.0
     PERIPHERAL  Likelihood = -1.44
     modified ALOM score:  -0.61
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 11
     IRSEIL
Discrimination of mitochondrial target seq.: 
     positive ( 1.84)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  8.90 at (39)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  1.04
     score from the 1-31 region:  9.70
Chloroplast protein?  Status: negative (-1.49)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 11)  from: 54  to: 72  Score:  7.5
Mitochondrial matrix?  Score:  0.40
Mitochondrial intermemb.space?  Score:  0.30
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: -1(213), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -2.34
Peroxisomal proteins?   Status: negative
Amino acid composition tendency for vacuolar proteins
     Score: -4.23  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 165 (3) HRKK
     Found: pos: 166 (3) RKKH
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.72
Nuclear Signal   Status: notclr ( 0.42)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

       mitochondrial matrix space --- Certainty= 0.569(Affirmative) < succ>
mitochondrial intermembrane space --- Certainty= 0.499(Affirmative) < succ>
                          nucleus --- Certainty= 0.420(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.284(Affirmative) < succ>


----- The End -----