Query Information

ORIGIN plant
BEGIN
>MYSEQ
 ARGTIHRTLP EEGYDAASYP RYEGRGHMHC IHMHSLKYLQ MMWFYTLRDM
 KIEYLHIAFL CIHSDICRYC RGEVLNLLWL FEEMRYWLYW DYVLYLQVFL
 ILIVILLKCL IQFVVISHQN NLAEMFHLTL ALLNAGACCS SRGVGCSSTS
 TFTLIIMLPR HSHDLIRNFL SKDVCFLTSR AHLIQFMLLW LSSHCIINVF
 YVFLSYLFIL CCIPVYAIFK GDDVEIKKKK 

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          230 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 100 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 107   Charge diffirence(C-N):  0.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   0
     Peak Value of UR:  -0.66
     Net Charge of CR: 2
     Discriminant Score:    -21.50
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -9.01
     Possible cleavage site: 48
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 2 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 2  value: -13.32  threshold: -2.0
     INTEGRAL    Likelihood =-13.32   Transmembrane  100 - 116 (  88 - 119)
     INTEGRAL    Likelihood = -7.70   Transmembrane  199 - 215 ( 193 - 221)
     PERIPHERAL  Likelihood =  0.85
     modified ALOM score:   2.76
>>> Likely a Type IIIa membrane protein (Ncyt Cexo)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 9
     HRTLPE
Discrimination of mitochondrial target seq.: 
     negative (-4.93)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.97 at (48)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  2.05
     score from the 1-31 region:  3.88
Chloroplast protein?  Status: negative (-5.99)

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.553
SKL motif (signal for peroxisomal protein): 
     pos: 181(230), count: 1   AHL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   5.44
Peroxisomal proteins?   Status: positive
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score:  1.70  Status: notclr
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 226 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.03)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.600(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.553(Affirmative) < succ>
                       Golgi body --- Certainty= 0.400(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.300(Affirmative) < succ>


----- The End -----