Query Information

ORIGIN plant
BEGIN
>MYSEQ
 ANLHRKIRRT TSMAINHKIS RRRPQLLSFC FWTGLSACLS LLLDMLLSMR
 PLCFDRDLTP LMVLFSYQLP GLRFSSVAYL IRAAEKRIPA GLARGKRLIL
 VFRFGSVSVM WPSSPIDYLS KLDYQIWISP RKHVCLISIH LQGTKASTIA
 DHIQNYSRKG LSSTIVGIAR FNWRRFVYNQ ILLLLNVMPM LDYLLCRGYR
 DFSDRKGSLS R

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          211 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 26 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 33   Charge diffirence(C-N): -6.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   7
     Peak Value of UR:   0.16
     Net Charge of CR: 5
     Discriminant Score:    -11.59
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 3.17
     Possible cleavage site: 38
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -2.07  threshold: -2.0
     INTEGRAL    Likelihood = -2.07   Transmembrane   26 -  42 (  26 -  43)
     PERIPHERAL  Likelihood = -0.69
     modified ALOM score:   0.51
>>> Seems to be a Type II (Ncyt Cexo) membrane protein
     The cytoplasmic tail is from 1 to 25 (25 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 25
     RRPQLL
Discrimination of mitochondrial target seq.: 
     positive ( 2.11)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  8.71 at (64)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -0.81
     score from the 1-31 region:  7.49
Chloroplast protein?  Status: negative (-5.60)

*** Reasoning Step: 2

Relative position of the cytoplasmic tail: 11%
     Larger value (>30%) is favared for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 25)  from: 26  to: 43  Score:  8.5
Mitochondrial inner membrane?  Score:  0.31
Mitochondrial intermemb.space?  Score:  0.09
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.103
SKL motif (signal for peroxisomal protein): 
     pos: 120(211), count: 1   SKL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   2.44
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.391
Checking the consensus for Golgi
Checking the consensus for Golgi
Checking the cytoplasmic tail of type II (Golgi)
Amino acid composition tendency for vacuolar proteins
     Score: -2.63  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:  20 (4) RRRP
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos:   7 (3) RR TTSMAINHKI SRRRP
Final Robbins Score (nucleus):  0.36
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.24)
Check mitochondrial signal for typeII (plasma memb.)
Type II is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.650(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.574(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.523(Affirmative) < succ>
mitochondrial intermembrane space --- Certainty= 0.373(Affirmative) < succ>


----- The End -----