Query Information

ORIGIN plant
BEGIN
>MYSEQ
 TTAWNGSRTR SGQSPFWRQM DSVCNLYRAS PNSRSNLSAP VSMERSASVQ
 SRRRLPPPES VCSMKKMEPQ LSSSKNSGAS ARKARRWHRR RRKMQRAVSR
 QHAAGTRICA ATRNASSKKS WLSCGGRAPG DAQALGIVSL LLTLPVYAVG
 LFYLCIAVGS DRCAVVTFVQ PFGI

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          174 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 140 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 147   Charge diffirence(C-N): -2.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   7
     Peak Value of UR:   0.41
     Net Charge of CR: 2
     Discriminant Score:    -11.85
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -5.5
     Possible cleavage site: 35
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -5.63  threshold: -2.0
     INTEGRAL    Likelihood = -5.63   Transmembrane  140 - 156 ( 132 - 160)
     PERIPHERAL  Likelihood =  5.89
     modified ALOM score:   1.23
>>> Seems to be a Type II (Ncyt Cexo) membrane protein
     The cytoplasmic tail is from 1 to 139 (139 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 56
     RRLPPP
Discrimination of mitochondrial target seq.: 
     notclr ( 0.98)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 10.58 at (35)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -0.84
     score from the 1-31 region:  9.25
Chloroplast protein?  Status: negative (-3.34)

*** Reasoning Step: 2

Relative position of the cytoplasmic tail: 79%
     Larger value (>30%) is favared for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 56)  from: 23  to: 24  Score:  1.5
Mitochondrial inner membrane?  Score:  0.49
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.245
SKL motif (signal for peroxisomal protein): 
     pos: -1(174), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -2.33
Peroxisomal proteins?   Status: negative
Checking the cytoplasmic tail of type II (Golgi)
Amino acid composition tendency for vacuolar proteins
     Score: -8.67  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:  87 (3) HRRR
     Found: pos:  88 (5) RRRR
     Found: pos:  89 (5) RRRK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.65
Nuclear Signal   Status: notclr ( 0.35)
Check mitochondrial signal for typeII (plasma memb.)
Type II is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

     mitochondrial inner membrane --- Certainty= 0.591(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.440(Affirmative) < succ>
                          nucleus --- Certainty= 0.350(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.245(Affirmative) < succ>


----- The End -----