Query Information

ORIGIN plant
BEGIN
>MYSEQ
 TILLGIQKVV VFMNSTNQFI QFFFLSYVKV GKKGTQIIFL ITSIVITRGF
 IFSGGFRFNL LFAVLCHEAL VADVGEEESL GDGDVGGVLV VGGVAALVGV
 PFATNMGIAA LLLVIPLLFL SSPLLVVTSV TVTRYRTFSN KMTGLTTLVA
 NLLGAGLVVF PPPLFEDLAE TLDDERHFLI VELGGVDWLS TWCSLLLLLL
 R

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          201 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 5
     Position of the most N-terminal TMS: 37 at i=3
MTOP: membrane topology (Hartmann et al.)
     I(middle): 44   Charge diffirence(C-N): -1.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   20
     Peak Value of UR:   1.90
     Net Charge of CR: 1
     Discriminant Score:      1.85
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -0.47
     Possible cleavage site: 49
>>> Seems to have an uncleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 5  value:  -9.87  threshold:  0.5
     INTEGRAL    Likelihood = -9.87   Transmembrane  111 - 127 (  94 - 133)
     INTEGRAL    Likelihood = -5.79   Transmembrane   87 - 103 (  82 - 106)
     INTEGRAL    Likelihood = -4.57   Transmembrane   37 -  53 (  35 -  54)
     INTEGRAL    Likelihood = -2.66   Transmembrane  144 - 160 ( 143 - 161)
     INTEGRAL    Likelihood = -0.37   Transmembrane   58 -  74 (  58 -  74)
     PERIPHERAL  Likelihood =  1.06
     modified ALOM score:   2.57
>>> Likely a Type IIIa membrane protein (Ncyt Cexo)
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 59
     FRFNLL
Discrimination of mitochondrial target seq.: 
     notclr ( 0.52)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  8.41 at (26)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.95
     score from the 1-31 region:  5.36
Chloroplast protein?  Status: negative (-6.05)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
Memb.protein with uncleavable signl is often at ER
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 59)  from: 37  to: 46  Score:  7.0
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: -1(201), count: 0   
Amino Acid Composition Tendency for Peroxisome:   6.89
Peroxisomal proteins?   Status: notclr
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score: -1.26  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.685(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.640(Affirmative) < succ>
                       Golgi body --- Certainty= 0.460(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>


----- The End -----