Query Information

ORIGIN plant
BEGIN
>MYSEQ
 QRTPSQNETG LPAIAAMSLE PFILENNKFM GNTTLKVWLF INHAYTTQSC
 TRNAPFEAYQ NLHSSRTSIF DLDHILHKYK LARHLDNLQD KFMRATYHLI
 LPDQVFGSSM FRKRNMHRKS SKRMRTVTIA RRARLLIRRG FRMKKTILNF
 FVSLVFLRSF KLSFFTTEVA DTQSSVKPLL QTTSRSLSFS LVVSLENLPE
 GHLIFYQEMV 

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          210 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   11
     Peak Value of UR:   1.70
     Net Charge of CR: 0
     Discriminant Score:     -2.96
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -1.98
     Possible cleavage site: 49
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   0.95  threshold: -2.0
     PERIPHERAL  Likelihood =  0.95
     modified ALOM score:  -1.09
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 4
     QRTPSQ
Discrimination of mitochondrial target seq.: 
     negative (-2.52)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  8.73 at (33)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  2.02
     score from the 1-31 region:  7.80
Chloroplast protein?  Status: negative (-2.44)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: 133(210), count: 1   ARL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   4.24
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.633
Amino acid composition tendency for vacuolar proteins
     Score: -4.83  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos: 117 (3) RK SSKRMRTVTI ARRAR
Final Robbins Score (nucleus):  0.60
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.70
Nuclear Signal   Status: notclr ( 0.40)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.760(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.743(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.100(Affirmative) < succ>


----- The End -----