Query Information

ORIGIN plant
BEGIN
>MYSEQ
 PTSIERFAEL PRWLSTTRSV DEDPNFSLSA FGLAAPACLF SWICFFRDRS
 ALIGILLHWF CFRTNCRAYA FRVPILSEER RRKGYPQGWL GGRDYWSFAS
 DLSRVCGPLL QSTTSVSTNT RSGKSDPRGS MFASLFDIYK ALKLQPPITR
 FRTTAVKDAA PLELALIGEG SYTTSKFYFY LMCPCWTICF AEDIDRISDR
 IGKVACLA

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          208 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 30 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 37   Charge diffirence(C-N):  3.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   2
     Peak Value of UR:  -0.05
     Net Charge of CR: -1
     Discriminant Score:    -18.61
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 0.38
     Possible cleavage site: 41
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -2.60  threshold: -2.0
     INTEGRAL    Likelihood = -2.60   Transmembrane   30 -  46 (  28 -  46)
     PERIPHERAL  Likelihood =  4.29
     modified ALOM score:   0.62
>>> Seems to be a Type Ib (Nexo Ccyt) membrane protein
     The cytoplasmic tail is from 47 to 208 (163 Residues)
 count: 1)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 70
     (count:1)
     CRAYAF
Discrimination of mitochondrial target seq.: 
     positive ( 2.11)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 11.19 at (64)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.03
     score from the 1-31 region:  8.62
Chloroplast protein?  Status: negative (-2.93)

*** Reasoning Step: 2

> Relative position of the end of the tail: 22%
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 70)  from: 26  to: 46  Score: 11.0
>>> Seems to have an intramitochondrial signal
Mitochondrial inner membrane?  Score:  0.37
Mitochondrial intermemb.space?  Score:  0.22
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.124
SKL motif (signal for peroxisomal protein): 
     pos: -1(208), count: 0   
Amino Acid Composition Tendency for Peroxisome:   7.11
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.640
Amino acid composition tendency for vacuolar proteins
     Score: -2.03  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:  79 (5) RRRK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.10)
Check cytoplasmic tail for typeIb (plasma memb.)
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

           microbody (peroxisome) --- Certainty= 0.640(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.600(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.567(Affirmative) < succ>
mitochondrial intermembrane space --- Certainty= 0.466(Affirmative) < succ>


----- The End -----