Query Information

ORIGIN plant
BEGIN
>MYSEQ
 PARGGSTALG GGSTALRQKI LPNGFAPTIY TTSYLSPQSN EHDLNSIFNP
 RNTSLFFSHI FCLSHFKSLE RNLVSLRAAV LCFISKSLLL FFFIRALVLH
 RVLCGFITLG ASSSTTRCFL VSKSCGRPQE SLYYPLVAKI SVAPLWSAKG
 GLGLKETRLF VGASTRKGTL VVPNLGIKKK KKKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          185 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 78 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 85   Charge diffirence(C-N):  0.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   13
     Peak Value of UR:   0.84
     Net Charge of CR: 1
     Discriminant Score:     -7.72
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -4.56
     Possible cleavage site: 18
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -7.06  threshold: -2.0
     INTEGRAL    Likelihood = -7.06   Transmembrane   78 -  94 (  73 - 100)
     PERIPHERAL  Likelihood = -0.06
     modified ALOM score:   1.51
>>> Seems to be a Type II (Ncyt Cexo) membrane protein
     The cytoplasmic tail is from 1 to 77 (77 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 19
     LRQKIL
Discrimination of mitochondrial target seq.: 
     negative (-2.47)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  8.08 at (67)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.09
     score from the 1-31 region:  7.12
Chloroplast protein?  Status: negative (-5.24)
Res.2 is Ala (a feature for chloroplast seq.)
Res.2 is Ala (a feature for chloroplast seq.)
Res.2 is Ala (a feature for chloroplast seq.)

*** Reasoning Step: 2

Relative position of the cytoplasmic tail: 41%
     Larger value (>30%) is favared for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.302
SKL motif (signal for peroxisomal protein): 
     pos: -1(185), count: 0   
Amino Acid Composition Tendency for Peroxisome:   3.08
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.477
Checking the cytoplasmic tail of type II (Golgi)
Amino acid composition tendency for vacuolar proteins
     Score:  1.76  Status: notclr
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 177 (5) KKKK
     Found: pos: 178 (5) KKKK
     Found: pos: 179 (5) KKKK
     Found: pos: 180 (5) KKKK
     Found: pos: 181 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos: 165 (5) RK GTLVVPNLGI KKKKK
Final Robbins Score (nucleus):  0.80
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.80
Nuclear Signal   Status: notclr ( 0.55)
Check mitochondrial signal for typeII (plasma memb.)
Type II is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                          nucleus --- Certainty= 0.910(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.850(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.477(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.442(Affirmative) < succ>


----- The End -----