Query Information

ORIGIN plant
BEGIN
>MYSEQ
 HNRWLDIQYP YVPNRLLTVM KNLEQNFDFQ NPMRNNSKPY LLYPFSYKSR
 GRDYFGGRIC NARNLILGNN NRNQSKVSLM NSYTLEPTIF KIETLKQYLR
 NRNRNSVCKF QEIMNGLSPS RTVYKIFFYV LKLMIEKKYI TSPNNQVMNI
 HFMKFLKYLK PYFLKNKHFM CALHINKFSN NEVRLVKKIL AYYTGYFICL
 LILLKSKTNK NSWDLKLKWE NKKHKKKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          229 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 188 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 195   Charge diffirence(C-N):  0.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   8
     Peak Value of UR:   1.17
     Net Charge of CR: 1
     Discriminant Score:     -6.88
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -3.24
     Possible cleavage site: 51
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -2.02  threshold: -2.0
     INTEGRAL    Likelihood = -2.02   Transmembrane  188 - 204 ( 188 - 205)
     PERIPHERAL  Likelihood =  6.58
     modified ALOM score:   0.50
>>> Seems to be a Type II (Ncyt Cexo) membrane protein
     The cytoplasmic tail is from 1 to 187 (187 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 17
     NRLLTV
Discrimination of mitochondrial target seq.: 
     negative (-2.21)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.42 at (68)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.03
     score from the 1-31 region:  2.61
Chloroplast protein?  Status: negative (-10.00)

*** Reasoning Step: 2

Relative position of the cytoplasmic tail: 81%
     Larger value (>30%) is favared for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.101
SKL motif (signal for peroxisomal protein): 
     pos: -1(229), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -0.51
Peroxisomal proteins?   Status: negative
Checking the cytoplasmic tail of type II (Golgi)
Amino acid composition tendency for vacuolar proteins
     Score:  1.63  Status: notclr
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 221 (3) KKHK
     Found: pos: 222 (3) KHKK
     Found: pos: 223 (3) HKKK
     Found: pos: 224 (5) KKKK
     Found: pos: 225 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.65
Nuclear Signal   Status: notclr ( 0.35)
Check mitochondrial signal for typeII (plasma memb.)
Type II is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.850(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.440(Affirmative) < succ>
                          nucleus --- Certainty= 0.350(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.101(Affirmative) < succ>


----- The End -----