Query Information

ORIGIN plant
BEGIN
>MYSEQ
 FRVIQAPRHV TRYPIFWVLR PRIQTSVWLI QIRVGRVQVQ VVQVWIMVQI
 MGILPTPWNT VQPILTWSHR AAQWIGNSRH RRQAPYQETV PYALIVQSVA
 TLVVPALQTV LVPLPSANRY SSLARRNQCI FLHHISYIRC SRRNSLRATG
 LHDRDAQVIA NLEPKLCKSF GIITSSMAIS EGRTHLQMFK HDESKGNSAV
 LTHGNLHNDE TVYRPFEPIQ GI

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          222 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 39 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 46   Charge diffirence(C-N):  2.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   2
     Peak Value of UR:   0.88
     Net Charge of CR: 3
     Discriminant Score:     -9.47
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -5.19
     Possible cleavage site: 25
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -2.13  threshold: -2.0
     INTEGRAL    Likelihood = -2.13   Transmembrane   98 - 114 (  98 - 115)
     PERIPHERAL  Likelihood = -0.96
     modified ALOM score:   0.53
>>> Seems to be a Type Ib (Nexo Ccyt) membrane protein
     The cytoplasmic tail is from 115 to 222 (109 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 149
     LRATGL
Discrimination of mitochondrial target seq.: 
     positive ( 2.26)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 13.12 at (30)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.03
     score from the 1-31 region:  1.57
Chloroplast protein?  Status: negative (-9.44)

*** Reasoning Step: 2

> Relative position of the end of the tail: 51%
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 149)  from: 50  to: 54  Score:  4.0
Mitochondrial inner membrane?  Score:  0.32
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.105
SKL motif (signal for peroxisomal protein): 
     pos: -1(222), count: 0   
Amino Acid Composition Tendency for Peroxisome:   1.53
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.270
Amino acid composition tendency for vacuolar proteins
     Score: -13.93  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:  78 (3) RHRR
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.10)
Check cytoplasmic tail for typeIb (plasma memb.)
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.700(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.538(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.326(Affirmative) < succ>
mitochondrial intermembrane space --- Certainty= 0.326(Affirmative) < succ>


----- The End -----