Query Information

ORIGIN plant
BEGIN
>MYSEQ
 AFFMASLFMI SLHVAIFEDL CISVDCVATS EVSVSVSLVP SKIWSRELIQ
 QASVSIPSLY QVEAHDKLFL VTCISKRNIF IPILCTSTLL TNFELDNVVE
 ELASRNTRDF FCKLDSPCLN FLQELFYCSS MCFTIVNNLS GTPCSRAENS
 NRSSTSCNTH FVICKYQRNI FSLCEFDLHA SSYVNTMPKN NKYILPNYAS
 CWILFCVHFV TRKYRPKKKK K

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          221 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 3
     Position of the most N-terminal TMS: 2 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 9   Charge diffirence(C-N): -3.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   11
     Peak Value of UR:   2.34
     Net Charge of CR: 0
     Discriminant Score:      1.49
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 1.76
     Possible cleavage site: 45
>>> Seems to have a cleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 46
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -2.23  threshold: -2.0
     INTEGRAL    Likelihood = -2.23   Transmembrane    2 -  18 (   1 -  18)
     PERIPHERAL  Likelihood =  0.10
     modified ALOM score:   0.55
>>> Seems to be a Type Ia membrane protein
     The cytoplasmic tail is from 19 to 221 (203 Residues)
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
(17) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 17
     Uncleavable? Ipos set to: 27
Discrimination of mitochondrial target seq.: 
     positive ( 1.68)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 13.48 at (27)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.95
     score from the 1-31 region:  9.08
Chloroplast protein?  Status: negative (-0.91)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 27)  from: 1  to: 17  Score:  9.0
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: -1(221), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -3.11
     AAC not from the N-term., score modified
Peroxisomal proteins?   Status: negative
Amino acid composition tendency for vacuolar proteins
     Score:  0.57  Status: notclr
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 214 (4) RPKK
     Found: pos: 215 (4) PKKK
     Found: pos: 216 (5) KKKK
     Found: pos: 217 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.05)
Type Ia is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.460(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.100(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>
                          outside --- Certainty= 0.100(Affirmative) < succ>


----- The End -----